Quote Originally Posted by RyougaZell View Post
The first Jinchuuriki looked too old to be Kushina's mother.
Besides... that woman married the 1st Hokage... who is Tsunade's grandfather... and Tsunade if over 50. So... no.
Not that I saw anything indicating that the Mito was Kushina's mother either but age based arguments don't necessarily hold since a point was made to state that...
Quote Originally Posted by Chapter 500 Page 03
The people of the Eddy Village were all blessed with astonishingly long lives
If the village members life spans were extended who's to say their child bearing years weren't also extended making it possible for them to have kids longer than normal. Mito apparently lived from the time of the founding until at least the time the Fourth was a kid.

That said from the way things were described it seems like Kushina knew of Mito as a respected elder but wasn't related to her by anything other than whatever distant bond related the members of their clan.